This heterogeneity plays a significant role in various biological processes including tumor progression and immune response. Phenotypically identical cells from the same population can have dramatic heterogeneity in their behavior. and the single cells of the eukaryotic Sphaerocystis sp. The protocol was directly applied to Gloeocapsa sp. We consider 80% of single-cell replicates that lead to >5 ng DNA after amplification as successful attempts. Our protocol is based on thermal and chemical lysis. as a gram-negative model due to major challenges reported in previous studies. Corynebacterium glutamicum was chosen as a typical gram-positive model and Nostoc sp.

In this work, we present a lysis protocol that can be used to extract genomic DNA from both gram-positive and gram-negative species without interfering with the amplification chemistry. The major challenges that bacterial species pose to genome amplification from single cells include the rigid bacterial cell walls and the need for an effective lysis protocol compatible with microfluidic platforms. So far, single-cell sequencing has been focused mostly on human cells due to the ease of lysing the cells for genome amplification. This technology is mostly coupled with microfluidic systems for controlled cell manipulation and precise fluid handling to shed light on the genomes of a wide range of cells.

Single-cell sequencing is a powerful technology that provides the capability of analyzing a single cell within a population.

Cell lysis buffer for dna extraction